The Laser Analytics Group develops advanced microscopic imaging techniques to probe molecular mechanisms of disease. In particular we have developed and maintain a number of so called optical superresolution microscopes (see superresolution link), which use physical principles and image reconstruction algorithms to achieve a spatial resolution down to 10 nanometres, ca 50 times smaller than the wavelength of the probing light. Our work makes extensive use of image processing and we are interested in inverse modelling problems, image deconvolution, denoising, object tracking, and various image reconstruction algorithms. We are very keen to collaborate with groups with compatible research interests.
The image above shows the resolution enhancement achievable using a technique called structured illumination microscopy (SIM). The technique uses spatially modulated illumination patterns to excite sample molecules, and subsequent fluorescence is collected in wide field mode on a CCD camera. Spatial frequency content in the object is 'mixed' with the illumination frequencies, and high frequency object information is thus encoded in low frequency 'Moire fringes', essentially beat patterns of excitation and sample spatial frequencies. This provides for a mode of transmitting high frequency information through a standard microscope system, and the high frequency content is recoverable from the Moire patterns using Fourier techniques and image reconstruction.
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