The ever-increasing speed and resolution of modern microscopes make the storage and post-processing of images challenging. We developed a 4-lens light sheet microscope combined with real-time image-processing engine that projects the 3D image volume onto a 2D map, reducing the data generated as well as providing a panoramic view of the sample. In my talk, I will discuss our multi-dimensional imaging approach, integrating whole-embryo live imaging, genetic information and analysis of an ensemble of specimen to understand how large-scale tissue movements shape the early zebrafish embryo.
Classical genetics has typically focused on dissecting how genes or pathways control a given process within cells. However, many genes likely play roles in multiple processes, which are potentially linked to one another. Unravelling those multiple roles and links is a pressing challenge if we are to understand how healthy cells function normally as integrated systems and, conversely, how complex cellular pathologies arise in disease and how to fix them.
A collaborative project between the Institute of Astronomy and the CRUK Cambridge Institute.
At the CRUK CI, we use an automated high-content microscope to image thousands of cells and then determine the percentage of mitotic cells with the associated commercial software.